Western Blot, Positive WB detected in: A549 whole cell lysate, All lanes: IKBKB antibody at 3.4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 87, 86, 30, 80 kDa, Observed band size: 87 kDa
IHC image of CAC14994 diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of A549 cells using CAC14994 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)