Western Blot, Positive WB detected in: HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue, All lanes: IDH3A antibody at 3.4ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 40, 32 kDa, Observed band size: 40 kDa
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC14959 at dilution of 1:100
Immunofluorescent analysis of A549 cells using CAC14959 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating IDH3A in HepG2 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC14959 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14959 (6ug) + HepG2 whole cell lysate (500ug), Lane 3: HepG2 whole cell lysate (20ug)