β-hydroxybutyryl-HIST1H3A (K56) Polyclonal Antibody, Cat#CAC15622
Immunofluorescence staining of Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) with CAC15622 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunocytochemistry analysis of CAC15622 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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β-hydroxybutyryl-HIST1H3A (K56) Polyclonal Antibody, Cat#CAC15622 Immunofluorescence staining of Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) with CAC15622 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunocytochemistry analysis of CAC15622 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

β-hydroxybutyryl-HIST1H3A (K56) Polyclonal Antibody

BiomatikSKU: CAC15622-100ul
Size: 100ul
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Immunofluorescence staining of Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) with CAC15622 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunocytochemistry analysis of CAC15622 diluted at 1:30 and staining in Hela cells (treated with 30mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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