β-hydroxybutyryl-HIST1H3A (K4) Polyclonal Antibody, Cat#CAC15569
Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 50mM sodium 3-hydroxybutyrate for 72h, All lanes: HIST1H3A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunocytochemistry analysis of CAC15569 diluted at 1:20 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium 3-hydroxybutyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15569) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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β-hydroxybutyryl-HIST1H3A (K4) Polyclonal Antibody, Cat#CAC15569 Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 50mM sodium 3-hydroxybutyrate for 72h, All lanes: HIST1H3A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa Immunocytochemistry analysis of CAC15569 diluted at 1:20 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium 3-hydroxybutyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15569) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

β-hydroxybutyryl-HIST1H3A (K4) Polyclonal Antibody

BiomatikSKU: CAC15569-100ul
Size: 100ul
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Western Blot, Detected samples: Hela whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 50mM sodium 3-hydroxybutyrate for 72h, All lanes: HIST1H3A antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunocytochemistry analysis of CAC15569 diluted at 1:20 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium 3-hydroxybutyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15569) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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