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Western Blot, Detected samples: A549 whole cell lysate; Untreated (-) or treated (+) with 50mM sodium 3-hydroxybutyrate for 72h, All lanes: HIST1H3A antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium 3-hydroxybutyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15573) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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