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Western Blot, Detected samples: 293 whole cell lysate, A549 whole cell lysate; Untreated (-) or treated (+) with 50mM Sodium 3-hydroxybutyrate for 72h, All lanes: HIST1H2BC antibody at 1:100, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunocytochemistry analysis of CAC15572 diluted at 1:40 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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