HTRA2 Polyclonal Antibody, Cat#CAC14730
IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CAC14730 at dilution of 1:100
Immunoprecipitating HTRA2 in MCF-7 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14730 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14730 (8ug) + MCF-7 whole cell lysate (500ug), Lane 3: MCF-7 whole cell lysate (20ug)
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HTRA2 Polyclonal Antibody, Cat#CAC14730 IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunohistochemistry of paraffin-embedded human pancreatic cancer using CAC14730 at dilution of 1:100 Immunoprecipitating HTRA2 in MCF-7 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14730 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14730 (8ug) + MCF-7 whole cell lysate (500ug), Lane 3: MCF-7 whole cell lysate (20ug)

Ultra Sensitive IHC

HTRA2 Polyclonal Antibody

BiomatikSKU: CAC14730-100ug
Size: 100ug
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IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC14730 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CAC14730 at dilution of 1:100
Immunoprecipitating HTRA2 in MCF-7 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14730 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14730 (8ug) + MCF-7 whole cell lysate (500ug), Lane 3: MCF-7 whole cell lysate (20ug)

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Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

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