Western Blot, Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, All lanes: HSD17B10 antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 27, 26 kDa, Observed band size: 27 kDa
Immunofluorescence staining of A549 cells with CAC14384 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HSD17B10 in 293T whole cell lysate, Lane 1: Rabbit control IgG instead of CAC14384 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14384 (8ug) + 293T whole cell lysate (500ug), Lane 3: 293T whole cell lysate (10ug)