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Western Blot, Positive WB detected in: NIH/3T3 cell acid extracts, Hela cell acid extracts, All lanes: HIST1H4A antibody at 2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunofluorescent analysis of Hela cells using CAC15174 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H4A (CAC15174) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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