HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15635
Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H4A antibody at 1.1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H4A in Rat spleen tissue, Lane 1: Rabbit control IgG instead of CAC15635 in Rat spleen tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15635 (5ug) + Rat spleen tissue (500ug), Lane 3: Rat spleen tissue (20ug)
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HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15635 Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H4A antibody at 1.1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating HIST1H4A in Rat spleen tissue, Lane 1: Rabbit control IgG instead of CAC15635 in Rat spleen tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15635 (5ug) + Rat spleen tissue (500ug), Lane 3: Rat spleen tissue (20ug)

Ultra Sensitive IHC

HIST1H4A (Ab-12) Polyclonal Antibody

BiomatikSKU: CAC15635-100ul
Size: 100ul
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Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H4A antibody at 1.1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC15635 diluted at 1:20 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H4A in Rat spleen tissue, Lane 1: Rabbit control IgG instead of CAC15635 in Rat spleen tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15635 (5ug) + Rat spleen tissue (500ug), Lane 3: Rat spleen tissue (20ug)

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