HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15486
IHC image of CAC15486 diluted at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15486 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue, All lanes: HIST1H4A antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15486 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)
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HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15486 IHC image of CAC15486 diluted at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CAC15486 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue, All lanes: HIST1H4A antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15486 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

Ultra Sensitive IHC

HIST1H4A (Ab-12) Polyclonal Antibody

BiomatikSKU: CAC15486-100ul
Size: 100ul
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IHC image of CAC15486 diluted at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15486 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue, All lanes: HIST1H4A antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15486 (8ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

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