HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15480
IHC image of CAC15480 diluted at 1:75 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15480) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate, All lanes: HIST1H4A antibody at 0.33ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15480 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15480 (3ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)
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HIST1H4A (Ab-12) Polyclonal Antibody, Cat#CAC15480 IHC image of CAC15480 diluted at 1:75 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15480) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate, All lanes: HIST1H4A antibody at 0.33ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15480 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15480 (3ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

Ultra Sensitive IHC

HIST1H4A (Ab-12) Polyclonal Antibody

BiomatikSKU: CAC15480-100ul
Size: 100ul
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IHC image of CAC15480 diluted at 1:75 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15480) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Western Blot, Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate, All lanes: HIST1H4A antibody at 0.33ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
Immunoprecipitating HIST1H4A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15480 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15480 (3ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

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