HIST1H4A (Ab-1) Polyclonal Antibody, Cat#CAC15396
Western Blot, Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Mouse kidney tissue, All lanes: HIST1H4A antibody at 0.29ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
IHC image of CAC15396 diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15396 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15396) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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HIST1H4A (Ab-1) Polyclonal Antibody, Cat#CAC15396 Western Blot, Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Mouse kidney tissue, All lanes: HIST1H4A antibody at 0.29ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa IHC image of CAC15396 diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CAC15396 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15396) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Ultra Sensitive IHC

HIST1H4A (Ab-1) Polyclonal Antibody

BiomatikSKU: CAC15396-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Mouse kidney tissue, All lanes: HIST1H4A antibody at 0.29ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 12 kDa, Observed band size: 12 kDa
IHC image of CAC15396 diluted at 1:50 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15396 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A (CAC15396) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

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