HIST1H3A (Ab-37) Polyclonal Antibody, Cat#CAC15584
Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H3A antibody at 0.59ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunofluorescence staining of MCF-7 cells with CAC15584 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15584) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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HIST1H3A (Ab-37) Polyclonal Antibody, Cat#CAC15584 Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H3A antibody at 0.59ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa Immunofluorescence staining of MCF-7 cells with CAC15584 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Chromatin Immunoprecipitation Hela (10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15584) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

HIST1H3A (Ab-37) Polyclonal Antibody

BiomatikSKU: CAC15584-100ul
Size: 100ul
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Western Blot, Positive WB detected in: 293 whole cell lysate, All lanes: HIST1H3A antibody at 0.59ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
Immunofluorescence staining of MCF-7 cells with CAC15584 at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Chromatin Immunoprecipitation Hela (10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A (CAC15584) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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