Ultra Sensitive IHC

HIST1H3A (Ab-27) Polyclonal Antibody

BiomatikSKU: CAC15643-100ul

Size: 100ul
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Description

Technical Documents

Images

IHC image of CAC15643 diluted at 1:20 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot, Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, All lanes: HIST1H3A antibody at 1.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa

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