HIST1H3A (Ab-122) Polyclonal Antibody, Cat#CAC15601
Western Blot, Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, All lanes: HIST1H3A antibody at 0.54ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H3A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15601 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15601 (5ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)
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HIST1H3A (Ab-122) Polyclonal Antibody, Cat#CAC15601 Western Blot, Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, All lanes: HIST1H3A antibody at 0.54ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating HIST1H3A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15601 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15601 (5ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

Ultra Sensitive IHC

HIST1H3A (Ab-122) Polyclonal Antibody

BiomatikSKU: CAC15601-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat liver tissue, All lanes: HIST1H3A antibody at 0.54ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 16 kDa, Observed band size: 16 kDa
IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC15601 diluted at 1:5 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIST1H3A in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15601 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15601 (5ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)

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