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Western Blot, Positive WB detected in: A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, All lanes: HIST1H2AG antibody at 1ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 15 kDa, Observed band size: 15 kDa
Immunoprecipitating HIST1H2AG in HepG2 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15620 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15620 (5ug) + HepG2 whole cell lysate (500ug), Lane 3: HepG2 whole cell lysate (20ug)

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