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Western blot; All lanes: HIF1AN antibody at 12µg/ml; Lane 1: Mouse heart tissue; Lane 2: Mouse skeletal muscle tissue; Lane 3: K562 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: HL60 whole cell lysate; Lane 6: 293T whole cell lysate; Lane 7: Jurkat whole cell lysate; Lane 8: A375 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 41 kDa; Observed band size: 41, 48 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CAC07088 at dilution of 1:100
Immunofluorescent analysis of Hela cells using CAC07088 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating HIF1AN in MCF-7 whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC07088 (8µg) + MCF-7 whole cell lysate (500µg); , Lane 3: MCF-7 whole cell lysate (10µg)

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