HEPACAM Polyclonal Antibody

BiomatikSKU: CAC15300-100ug

Size: 100ug
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Technical Documents

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Western Blot, Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, All lanes: HEPACAM antibody at 3.2ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 47, 41 kDa, Observed band size: 47 kDa
Immunofluorescence staining of MCF-7 cells with CAC15300 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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