Western Blot, All lanes: HARS antibody at 6ug/ml, Lane 1: Hela whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: 293T whole cell lysate, Secondary, Goat polyclonal to rabbit IgG at 1/10000 dilution, Predicted band size: 58, 53, 51, 55 kDa, Observed band size: 58 kDa
Immunohistochemistry of paraffin-embedded human colon tissue using CAC13060 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CAC13060 at dilution of 1:100
Immunoprecipitating HARS in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of (1ug) instead of CAC13060 in Hela whole cell lysate., For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000), Lane 2: CAC13060 (4ug) + Hela whole cell lysate (500ug), Lane 3: Hela whole cell lysate (20ug)