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IHC image of CAC08496 diluted at 1:350 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of HepG2 cells using CAC08496 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue; , All lanes: GPT antibody at 4.5µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 55 kDa; , Observed band size: 55 kDa
IHC image diluted at 1:350 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Safety Data Sheet