Description
Technical Documents
Images
Immunofluorescence staining of MCF-7 cells with CAC09689 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot; ,Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate; ,All lanes: GPA33 antibody at 3.2µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 36 kDa; ,Observed band size: 50 kDa
Immunohistochemistry of paraffin-embedded human small intestine tissue using CAC09689 at dilution of 1:100

Safety Data Sheet