GEMIN2 Polyclonal Antibody, Cat#CAC15697
Western Blot, Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, All lanes: GEMIN2 antibody at 3.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 32, 30, 29, 5 kDa, Observed band size: 32 kDa
Immunofluorescence staining of Hela cells with CAC15697 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating GEMIN2 in 293T whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15697 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC15697 (6ug) + 293T whole cell lysate (0.5mg), Lane 3: 293T whole cell lysate (20ug)
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GEMIN2 Polyclonal Antibody, Cat#CAC15697 Western Blot, Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, All lanes: GEMIN2 antibody at 3.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 32, 30, 29, 5 kDa, Observed band size: 32 kDa Immunofluorescence staining of Hela cells with CAC15697 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating GEMIN2 in 293T whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15697 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC15697 (6ug) + 293T whole cell lysate (0.5mg), Lane 3: 293T whole cell lysate (20ug)

GEMIN2 Polyclonal Antibody

BiomatikSKU: CAC15697-100ug
Size: 100ug
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Western Blot, Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, All lanes: GEMIN2 antibody at 3.7ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 32, 30, 29, 5 kDa, Observed band size: 32 kDa
Immunofluorescence staining of Hela cells with CAC15697 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating GEMIN2 in 293T whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15697 in 293T whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC15697 (6ug) + 293T whole cell lysate (0.5mg), Lane 3: 293T whole cell lysate (20ug)

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