Gdf5 Polyclonal Antibody, Cat#CAC10562
Immunohistochemistry of paraffin-embedded human ovarian cancer using CAC10562 at dilution of 1:100
Western Blot; ,Positive WB detected in: NIH/3T3 whole cell lysate; ,All lanes: GDF5 antibody at 2.64µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 56 kDa; ,Observed band size: 56 kDa;
IHC image diluted at 1:132 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:132 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Gdf5 Polyclonal Antibody, Cat#CAC10562 Immunohistochemistry of paraffin-embedded human ovarian cancer using CAC10562 at dilution of 1:100 Western Blot; ,Positive WB detected in: NIH/3T3 whole cell lysate; ,All lanes: GDF5 antibody at 2.64µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 56 kDa; ,Observed band size: 56 kDa; IHC image diluted at 1:132 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image diluted at 1:132 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Ultra Sensitive IHC

Gdf5 Polyclonal Antibody

BiomatikSKU: CAC10562-100ul
Size: 100ul
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CAC10562 at dilution of 1:100
Western Blot; ,Positive WB detected in: NIH/3T3 whole cell lysate; ,All lanes: GDF5 antibody at 2.64µg/ml; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/50000 dilution; ,Predicted band size: 56 kDa; ,Observed band size: 56 kDa;
IHC image diluted at 1:132 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image diluted at 1:132 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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