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Western Blot; Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate; All lanes: GBA antibody at 3.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 60, 58, 30, 51, 55 kDa; Observed band size: 51 kDa
IHC image diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Safety Data Sheet