Western Blot, Positive WB detected in: U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue, All lanes: GARS antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 84 kDa, Observed band size: 84 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using CAC14725 at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CAC14725 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating GARS in HL60 whole cell lysate, Lane 1: Rabbit control IgG instead of (1ug) instead of CAC14725 in HL60 whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14725 (8ug) + HL60 whole cell lysate (500ug), Lane 3: HL60 whole cell lysate (20ug)