Western blot; All lanes: FBXO4 antibody at 3.1µg/ml; Lane 1: 293T whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: HL60 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 45, 36 kDa; Observed band size: 45 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CAC10844 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CAC10844 at dilution of 1:100
Immunoprecipitating FBXO4 in HepG2 whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000); , Lane 2: CAC10844 (4µg) + HepG2 whole cell lysate (500µg); , Lane 3: HepG2 whole cell lysate (20µg)