Ultra Sensitive IHC

FABP4 Polyclonal Antibody

BiomatikSKU: CAC15864-100ul

Size: 100ul
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Technical Documents

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Western Blot, Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, All lanes: FABP4 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 15 kDa, Observed band size: 15 kDa
IHC image of CAC15864 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

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