EXOC7 Polyclonal Antibody, Cat#CAC15775
IHC image of CAC15775 diluted at 1:560 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CAC15775 at 1:186, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, All lanes: EXOC7 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 84, 79, 75, 33, 78, 81 kDa, Observed band size: 75 kDa
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EXOC7 Polyclonal Antibody, Cat#CAC15775 IHC image of CAC15775 diluted at 1:560 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CAC15775 at 1:186, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, All lanes: EXOC7 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 84, 79, 75, 33, 78, 81 kDa, Observed band size: 75 kDa

EXOC7 Polyclonal Antibody

BiomatikSKU: CAC15775-100ul
Size: 100ul
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IHC image of CAC15775 diluted at 1:560 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CAC15775 at 1:186, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate, All lanes: EXOC7 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 84, 79, 75, 33, 78, 81 kDa, Observed band size: 75 kDa

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