Esr1 Polyclonal Antibody

BiomatikSKU: CAC07157-100ug

Size: 100ug
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Technical Documents

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Western blot; All lanes: ESR1 antibody at 7µg/ml; Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: Rat brain tissue; Lane 4: Colo320 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 67, 54, 48, 36 kDa; Observed band size: 67 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CAC07157 at dilution of 1:100
Chromatin Immunoprecipitation MCF-7 (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter.

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