Emc8 Polyclonal Antibody, Cat#CAC08327
Immunofluorescence staining of MCF-7 cells with CAC08327 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: EMC8 antibody at 4µg/ml; ,Lane 1: A549 whole cell lysate; ,Lane 2: Human placenta tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HT29 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 24, 14 kDa; ,Observed band size: 24 kDa
Immunohistochemistry of paraffin-embedded human cervical cancer using CAC08327 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC08327 at dilution of 1:100
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Emc8 Polyclonal Antibody, Cat#CAC08327 Immunofluorescence staining of MCF-7 cells with CAC08327 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western blot; ,All lanes: EMC8 antibody at 4µg/ml; ,Lane 1: A549 whole cell lysate; ,Lane 2: Human placenta tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HT29 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 24, 14 kDa; ,Observed band size: 24 kDa Immunohistochemistry of paraffin-embedded human cervical cancer using CAC08327 at dilution of 1:100 Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC08327 at dilution of 1:100

Emc8 Polyclonal Antibody

BiomatikSKU: CAC08327-100ug
Size: 100ug
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Immunofluorescence staining of MCF-7 cells with CAC08327 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: EMC8 antibody at 4µg/ml; ,Lane 1: A549 whole cell lysate; ,Lane 2: Human placenta tissue; ,Lane 3: Hela whole cell lysate; ,Lane 4: HT29 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 24, 14 kDa; ,Observed band size: 24 kDa
Immunohistochemistry of paraffin-embedded human cervical cancer using CAC08327 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC08327 at dilution of 1:100

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