Ect2 Polyclonal Antibody, Cat#CAC11271
IHC image of CAC11271 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ECT2 in Hela whole cell lysate; ,Lane 1: Rabbit control IgG instead in Hela whole cell lysate.,For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000); ,Lane 2: CAC11271 (6µg) + Hela whole cell lysate (1mg); ,Lane 3: Hela whole cell lysate (20µg);
Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate; , All lanes:ECT2 antibody at 2.7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 104, 100, 109, 101 kDa; , Observed band size: 104 kDa;
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Ect2 Polyclonal Antibody, Cat#CAC11271 IHC image of CAC11271 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating ECT2 in Hela whole cell lysate; ,Lane 1: Rabbit control IgG instead in Hela whole cell lysate.,For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000); ,Lane 2: CAC11271 (6µg) + Hela whole cell lysate (1mg); ,Lane 3: Hela whole cell lysate (20µg); Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate; , All lanes:ECT2 antibody at 2.7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 104, 100, 109, 101 kDa; , Observed band size: 104 kDa;

Ultra Sensitive IHC

Ect2 Polyclonal Antibody

BiomatikSKU: CAC11271-100ug
Size: 100ug
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IHC image of CAC11271 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating ECT2 in Hela whole cell lysate; ,Lane 1: Rabbit control IgG instead in Hela whole cell lysate.,For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000); ,Lane 2: CAC11271 (6µg) + Hela whole cell lysate (1mg); ,Lane 3: Hela whole cell lysate (20µg);
Western Blot; , Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate; , All lanes:ECT2 antibody at 2.7µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 104, 100, 109, 101 kDa; , Observed band size: 104 kDa;

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I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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