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Immunofluorescence staining of A549 cells with CAC08374 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: DOK5 antibody at 2µg/ml + Mouse kidney tissue; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/15000 dilution; ,Predicted band size: 36, 23 kDa; ,Observed band size: 36 kDa
Immunohistochemistry of paraffin-embedded human tonsil tissue using CAC08374 at dilution of 1:100

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