Western Blot, Positive WB detected in: Rat heart tissue, All lanes: DLG2 antibody at 2.8ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 98, 110, 84, 101, 39 kDa, Observed band size: 98 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CAC15074 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CAC15074 at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CAC15074 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).