Di-methyl-HIST1H1C (K45) Polyclonal Antibody, Cat#CAC15640
Western Blot, Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa
Immunocytochemistry analysis of CAC15640 diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15640) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunoprecipitating HIST1H1C in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15640 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15640 (5ug) + Hela whole cell lysate (1mg), Lane 3: Hela whole cell lysate (20ug)
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Di-methyl-HIST1H1C (K45) Polyclonal Antibody, Cat#CAC15640 Western Blot, Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa Immunocytochemistry analysis of CAC15640 diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15640) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter. Immunoprecipitating HIST1H1C in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15640 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15640 (5ug) + Hela whole cell lysate (1mg), Lane 3: Hela whole cell lysate (20ug)

Di-methyl-HIST1H1C (K45) Polyclonal Antibody

BiomatikSKU: CAC15640-100ul
Size: 100ul
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Western Blot, Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, All lanes: HIST1H1C antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa
Immunocytochemistry analysis of CAC15640 diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Chromatin Immunoprecipitation Hela (4*10 6 ) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1C (CAC15640) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Immunoprecipitating HIST1H1C in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15640 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15640 (5ug) + Hela whole cell lysate (1mg), Lane 3: Hela whole cell lysate (20ug)

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