Ddx39B Polyclonal Antibody

BiomatikSKU: CAC08265-100ug

Size: 100ug
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Technical Documents

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Western blot; All lanes: DDX39B antibody at 2µg/ml; Lane 1: EC109 whole cell lysate; Lane 2: 293T whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/15000 dilution; Predicted band size: 49, 51 kDa; Observed band size: 49 kDa
Immunohistochemistry of paraffin-embedded human prostate cancer using CAC08265 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CAC08265 at dilution of 1:100
Immunofluorescence staining of MCF-7 cells with CAC08265 at 1:120, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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