Cstb Polyclonal Antibody, Cat#CAC08250
Immunohistochemistry of paraffin-embedded human colon cancer using CAC08250 at dilution of 1:100
IHC image diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate; , All lanes: CSTB antibody at 5.36µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 12 kDa; , Observed band size: 12 kDa
Immunofluorescence staining of HepG2 cells with CAC08250 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Cstb Polyclonal Antibody, Cat#CAC08250 Immunohistochemistry of paraffin-embedded human colon cancer using CAC08250 at dilution of 1:100 IHC image diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western Blot; , Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate; , All lanes: CSTB antibody at 5.36µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 12 kDa; , Observed band size: 12 kDa Immunofluorescence staining of HepG2 cells with CAC08250 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Ultra Sensitive IHC

Cstb Polyclonal Antibody

BiomatikSKU: CAC08250-100ug
Size: 100ug
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Immunohistochemistry of paraffin-embedded human colon cancer using CAC08250 at dilution of 1:100
IHC image diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot; , Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, 293T whole cell lysate; , All lanes: CSTB antibody at 5.36µg/ml; , Secondary; , Goat polyclonal to rabbit IgG at 1/50000 dilution; , Predicted band size: 12 kDa; , Observed band size: 12 kDa
Immunofluorescence staining of HepG2 cells with CAC08250 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

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