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Western Blot, Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate, All lanes: CSNK2A3 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 46 kDa, Observed band size: 46 kDa
IHC image of CAC13266 diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating CSNK2A3 in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of CAC13266 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: CAC13266 (5ug) + Hela whole cell lysate (0.5mg), Lane 3: Hela whole cell lysate (20ug)

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