Csnk1D Polyclonal Antibody, Cat#CAC08341
Western Blot; Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue; All lanes: CSNK1D antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 47 kDa; Observed band size: 48 kDa
IHC image diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC08341 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CSNK1D in Hela whole cell lysate; , Lane 1: Rabbit control IgG (1µg) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08341 (6µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)
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Csnk1D Polyclonal Antibody, Cat#CAC08341 Western Blot; Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue; All lanes: CSNK1D antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 47 kDa; Observed band size: 48 kDa IHC image diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CAC08341 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating CSNK1D in Hela whole cell lysate; , Lane 1: Rabbit control IgG (1µg) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08341 (6µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)

Ultra Sensitive IHC

Csnk1D Polyclonal Antibody

BiomatikSKU: CAC08341-100ug
Size: 100ug
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Western Blot; Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue; All lanes: CSNK1D antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 48, 47 kDa; Observed band size: 48 kDa
IHC image diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC08341 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating CSNK1D in Hela whole cell lysate; , Lane 1: Rabbit control IgG (1µg) instead in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: CAC08341 (6µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (10µg)

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