Description
Technical Documents
Images
Immunofluorescence staining of A549 cells with CAC08336 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: CRYBA1 antibody at 6µg/ml; ,Lane 1: Human placenta tissue; ,Lane 2: U251 whole cell lysate; ,Lane 3: U937 whole cell lysate; ,Lane 4: Mouse stomach tissue; ,Lane 5: Mouse liver tissue; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 26, 24 kDa; ,Observed band size: 24 kDa
Immunohistochemistry of paraffin-embedded human heart tissue using CAC08336 at dilution of 1:100

Safety Data Sheet