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Western Blot; Detected samples: K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM Sodium crotonylate for 4h; All lanes: HIST1H2BC antibody at 1:100; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14 kDa; Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC11528 at 1:12.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Safety Data Sheet