Crotonyl-HIST1H2BC (K12) Polyclonal Antibody, Cat#CAC15494
Western Blot, Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h), All lanes: HIST1H2BC antibody at 0.97ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15494 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H2BC in 293 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15494 in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15494 (5ug) + 293 whole cell lysate (500ug), Lane 3: 293 whole cell lysate (20ug)
Chromatin Immunoprecipitation Hela (10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15494) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Crotonyl-HIST1H2BC (K12) Polyclonal Antibody, Cat#CAC15494 Western Blot, Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h), All lanes: HIST1H2BC antibody at 0.97ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15494 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunoprecipitating HIST1H2BC in 293 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15494 in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15494 (5ug) + 293 whole cell lysate (500ug), Lane 3: 293 whole cell lysate (20ug) Chromatin Immunoprecipitation Hela (10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15494) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Crotonyl-HIST1H2BC (K12) Polyclonal Antibody

BiomatikSKU: CAC15494-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h), All lanes: HIST1H2BC antibody at 0.97ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CAC15494 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H2BC in 293 whole cell lysate, Lane 1: Rabbit control IgG instead of CAC15494 in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC15494 (5ug) + 293 whole cell lysate (500ug), Lane 3: 293 whole cell lysate (20ug)
Chromatin Immunoprecipitation Hela (10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H2BC (CAC15494) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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