Crotonyl-HIST1H2AG (K125) Polyclonal Antibody

BiomatikSKU: CAC15510-100ul

Size: 100ul
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Technical Documents

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Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) using CAC15510 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8ug anti-HIST1H2AG (CAC15510) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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