Western Blot; Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue; All lanes: CPNE7 antibody at 3.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 71, 62 kDa; Observed band size: 62 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.