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Western blot; All lanes: COMMD1 antibody at 5.36µg/ml; Lane 1: A549 whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: MCF-7 whole cell lysate; Lane 4: Hela whole cell lysate; Lane 5: HepG2 whole cell lysate; Secondary; Goat polyclonal to rabbit IgG at 1/10000 dilution; Predicted band size: 22, 18 kDa; Observed band size: 22 kDa
Immunohistochemistry of paraffin-embedded human liver cancer using CAC10746 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CAC10746 at dilution of 1:100
Immunoprecipitating COMMD1 in HepG2 whole cell lysate; , Lane 1: Rabbit control IgG instead of (1µg) instead in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000); , Lane 2: CAC10746 (4µg) + HepG2 whole cell lysate (500µg); , Lane 3: HepG2 whole cell lysate (20µg)

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