CDK5RAP3 Polyclonal Antibody

BiomatikSKU: CAC15827-100ul

Size: 100ul
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Technical Documents

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Western Blot, Positive WB detected in: U87 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue, All lanes: CDK5RAP3 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 57, 47, 32, 60 kDa, Observed band size: 57 kDa
Immunofluorescence staining of HepG2 cells with CAC15827 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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