CD48 Monoclonal Antibody, Cat#CAC13701
Western Blot, Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue , All lanes: CD48 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min
Western Blot, Positive WB detected in: 20ug Raji whole cell lysate , CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min
Immunofluorescence staining of JK cells with CAC13701 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay Peak curve showing JK cells stained with CAC13701 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CD48 Monoclonal Antibody, Cat#CAC13701 Western Blot, Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue , All lanes: CD48 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min Western Blot, Positive WB detected in: 20ug Raji whole cell lysate , CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min Immunofluorescence staining of JK cells with CAC13701 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). Overlay Peak curve showing JK cells stained with CAC13701 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

CD48 Monoclonal Antibody

BiomatikSKU: CAC13701-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue , All lanes: CD48 antibody at 1:2000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min
Western Blot, Positive WB detected in: 20ug Raji whole cell lysate , CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 28, 20 KDa, Observed band size: 43 KDa, Exposure time：5min
Immunofluorescence staining of JK cells with CAC13701 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay Peak curve showing JK cells stained with CAC13701 (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10 6 cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10 6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

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