Immunohistochemistry of paraffin-embedded human cervical cancer using CAC12894 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CAC12894 at dilution of 1:100
Western Blot, Positive WB detected in: NIH/3T3 whole cell lysate, All lanes: CCND2 antibody at 2.25ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 34 kDa, Observed band size: 34 kDa
IHC image of CAC12894 diluted at 1:225 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.