Capn1 Polyclonal Antibody, Cat#CAC07490
Immunofluorescence staining of MCF-7 cells with CAC07490 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: CAPN1 antibody at 6µg/ml; ,Lane 1: A375 whole cell lysate; ,Lane 2: 293T whole cell lysate; ,Lane 3: PC-3 whole cell lysate; ,Lane 4: Jurkat whole cell lysate; ,Lane 5: Hela whole cell lysate; ,Lane 6: A2780 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 82 kDa; ,Observed band size: 82 kDa
Immunohistochemistry of paraffin-embedded human heart tissue using CAC07490 at dilution of 1:100
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Capn1 Polyclonal Antibody, Cat#CAC07490 Immunofluorescence staining of MCF-7 cells with CAC07490 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western blot; ,All lanes: CAPN1 antibody at 6µg/ml; ,Lane 1: A375 whole cell lysate; ,Lane 2: 293T whole cell lysate; ,Lane 3: PC-3 whole cell lysate; ,Lane 4: Jurkat whole cell lysate; ,Lane 5: Hela whole cell lysate; ,Lane 6: A2780 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 82 kDa; ,Observed band size: 82 kDa Immunohistochemistry of paraffin-embedded human heart tissue using CAC07490 at dilution of 1:100

Capn1 Polyclonal Antibody

BiomatikSKU: CAC07490-100ug
Size: 100ug
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Immunofluorescence staining of MCF-7 cells with CAC07490 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot; ,All lanes: CAPN1 antibody at 6µg/ml; ,Lane 1: A375 whole cell lysate; ,Lane 2: 293T whole cell lysate; ,Lane 3: PC-3 whole cell lysate; ,Lane 4: Jurkat whole cell lysate; ,Lane 5: Hela whole cell lysate; ,Lane 6: A2780 whole cell lysate; ,Secondary; ,Goat polyclonal to rabbit IgG at 1/10000 dilution; ,Predicted band size: 82 kDa; ,Observed band size: 82 kDa
Immunohistochemistry of paraffin-embedded human heart tissue using CAC07490 at dilution of 1:100

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