Western Blot, Positive WB detected in: Jurkat whole cell lysate, HEK293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate, All lanes: CAMLG antibody at 3ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 33 kDa, Observed band size: 33 kDa
Immunofluorescent analysis of HepG2 cells using CAC14954 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitating CAMLG in HEK293 whole cell lysate, Lane 1: Rabbit control IgG (1ug) instead of CAC14954 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000), Lane 2: CAC14954 (8ug) + HEK293 whole cell lysate (500ug), Lane 3: HEK293 whole cell lysate (10ug)